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cat 7074  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc cat 7074
    Cat 7074, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cat+7074/pmc13018958-108-15-18
    Average 86 stars, based on 1 article reviews
    cat 7074 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Membrane:

    Article Title: UCHL1 promotes temozolomide resistance in glioblastoma by inhibiting the ubiquitination-mediated degradation of keratin 8.
    Article Snippet: .. After washing with TBST, the membrane was incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (Cat7074 and #7076, Cell Signaling Technology, Danvers, MA, USA) for 1 h. Protein bands were detected using an ECL detection system (Fude Biology, China), with GAPDH serving as a loading control. .. The primary antibodies used included UCHL1 (Cat#13179, Cell Signaling Technology), KRT8 (Cat#AG2433, Beyotime and Cat# bsm-52419R, Bioss, Beijing, China), PARP (Cat#9542, Cell Signaling Technology), Myc Tag (Cat#2276, Cell Signaling Technology), DYKDDDDK Tag (Cat#14793, Cell Signaling Technology), HA Tag (Cat#3724, Cell Signaling Technology), GAPDH (Cat#60004-1-Ig, Proteintech, Wuhan, China), γ-H2AX (Cat#9718, Cell Signaling Technology) and Bcl-2 (Cat#3498, Cell Signaling Technology).

    Article Title: UCHL1 promotes temozolomide resistance in glioblastoma by inhibiting the ubiquitination-mediated degradation of keratin 8
    Article Snippet: .. After washing with TBST, the membrane was incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (Cat7074 and #7076, Cell Signaling Technology, Danvers, MA, USA) for 1 h. Protein bands were detected using an ECL detection system (Fude Biology, China), with GAPDH serving as a loading control. .. The primary antibodies used included UCHL1 (Cat#13179, Cell Signaling Technology), KRT8 (Cat#AG2433, Beyotime and Cat# bsm-52419R, Bioss, Beijing, China), PARP (Cat#9542, Cell Signaling Technology), Myc Tag (Cat#2276, Cell Signaling Technology), DYKDDDDK Tag (Cat#14793, Cell Signaling Technology), HA Tag (Cat#3724, Cell Signaling Technology), GAPDH (Cat#60004-1-Ig, Proteintech, Wuhan, China), γ-H2AX (Cat#9718, Cell Signaling Technology) and Bcl-2 (Cat#3498, Cell Signaling Technology).

    Incubation:

    Article Title: UCHL1 promotes temozolomide resistance in glioblastoma by inhibiting the ubiquitination-mediated degradation of keratin 8.
    Article Snippet: .. After washing with TBST, the membrane was incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (Cat7074 and #7076, Cell Signaling Technology, Danvers, MA, USA) for 1 h. Protein bands were detected using an ECL detection system (Fude Biology, China), with GAPDH serving as a loading control. .. The primary antibodies used included UCHL1 (Cat#13179, Cell Signaling Technology), KRT8 (Cat#AG2433, Beyotime and Cat# bsm-52419R, Bioss, Beijing, China), PARP (Cat#9542, Cell Signaling Technology), Myc Tag (Cat#2276, Cell Signaling Technology), DYKDDDDK Tag (Cat#14793, Cell Signaling Technology), HA Tag (Cat#3724, Cell Signaling Technology), GAPDH (Cat#60004-1-Ig, Proteintech, Wuhan, China), γ-H2AX (Cat#9718, Cell Signaling Technology) and Bcl-2 (Cat#3498, Cell Signaling Technology).

    Article Title: UCHL1 promotes temozolomide resistance in glioblastoma by inhibiting the ubiquitination-mediated degradation of keratin 8
    Article Snippet: .. After washing with TBST, the membrane was incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (Cat7074 and #7076, Cell Signaling Technology, Danvers, MA, USA) for 1 h. Protein bands were detected using an ECL detection system (Fude Biology, China), with GAPDH serving as a loading control. .. The primary antibodies used included UCHL1 (Cat#13179, Cell Signaling Technology), KRT8 (Cat#AG2433, Beyotime and Cat# bsm-52419R, Bioss, Beijing, China), PARP (Cat#9542, Cell Signaling Technology), Myc Tag (Cat#2276, Cell Signaling Technology), DYKDDDDK Tag (Cat#14793, Cell Signaling Technology), HA Tag (Cat#3724, Cell Signaling Technology), GAPDH (Cat#60004-1-Ig, Proteintech, Wuhan, China), γ-H2AX (Cat#9718, Cell Signaling Technology) and Bcl-2 (Cat#3498, Cell Signaling Technology).

    Article Title: Astrocytic SIRT3 Alleviates Neuroinflammatory Responses After Cerebral Ischemia/reperfusion by Inhibiting the cGAS-STING Pathway
    Article Snippet: Equal amounts of proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, Thermo Fisher Scientific) and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Boston, MA, USA).The membranes were then blocked with 5% non-fat milk for 2 h at room temperature, followed by overnight incubation at 4 °C with primary antibodies against: SIRT3, cGAS, STING, p-STING, IRF3, p-IRF3 (Rabbit, 1:1000, Cell Signaling Technology), and iNOS (1:1000, Abcam). .. Subsequently, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5000, Cat 7074 and 7076, Cell Signaling Technology) for 1 h at room temperature. .. Protein bands were visualized using an enhanced chemiluminescence kit (Abbkine, Wuhan, China) and imaged with a Tanon imaging system.

    Article Title: Jumonji domain-containing 6 promotes the expansion of neuroblastoma stem cells by activating the wingless/ integrated pathway
    Article Snippet: The membranes were blocked using 5% non-fat milk and probed with the following primary antibodies: anti-JMJD6 antibody (1:1000, cat. 60602, CST, Danvers, MA, USA), anti-β-Catenin antibody (1:2000, cat. 8480, CST), anti-EF-1α antibody (1:2000, cat. sc-21758, Santa Cruz, Dallas, TX, USA), and anti-GAPDH antibody (1:10000, cat. 2118, CST). .. Then, the membranes were incubated with an horseradish peroxidase-conjugated second antibody (1:10000, cat. 7074, or 1:10000, cat. 7076, CST), and the bands were visualized with electrochemiluminescence luminescence (Bio-Rad, Hercules, CA, USA). ..

    Control:

    Article Title: UCHL1 promotes temozolomide resistance in glioblastoma by inhibiting the ubiquitination-mediated degradation of keratin 8.
    Article Snippet: .. After washing with TBST, the membrane was incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (Cat7074 and #7076, Cell Signaling Technology, Danvers, MA, USA) for 1 h. Protein bands were detected using an ECL detection system (Fude Biology, China), with GAPDH serving as a loading control. .. The primary antibodies used included UCHL1 (Cat#13179, Cell Signaling Technology), KRT8 (Cat#AG2433, Beyotime and Cat# bsm-52419R, Bioss, Beijing, China), PARP (Cat#9542, Cell Signaling Technology), Myc Tag (Cat#2276, Cell Signaling Technology), DYKDDDDK Tag (Cat#14793, Cell Signaling Technology), HA Tag (Cat#3724, Cell Signaling Technology), GAPDH (Cat#60004-1-Ig, Proteintech, Wuhan, China), γ-H2AX (Cat#9718, Cell Signaling Technology) and Bcl-2 (Cat#3498, Cell Signaling Technology).

    Article Title: UCHL1 promotes temozolomide resistance in glioblastoma by inhibiting the ubiquitination-mediated degradation of keratin 8
    Article Snippet: .. After washing with TBST, the membrane was incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (Cat7074 and #7076, Cell Signaling Technology, Danvers, MA, USA) for 1 h. Protein bands were detected using an ECL detection system (Fude Biology, China), with GAPDH serving as a loading control. .. The primary antibodies used included UCHL1 (Cat#13179, Cell Signaling Technology), KRT8 (Cat#AG2433, Beyotime and Cat# bsm-52419R, Bioss, Beijing, China), PARP (Cat#9542, Cell Signaling Technology), Myc Tag (Cat#2276, Cell Signaling Technology), DYKDDDDK Tag (Cat#14793, Cell Signaling Technology), HA Tag (Cat#3724, Cell Signaling Technology), GAPDH (Cat#60004-1-Ig, Proteintech, Wuhan, China), γ-H2AX (Cat#9718, Cell Signaling Technology) and Bcl-2 (Cat#3498, Cell Signaling Technology).

    Electrochemiluminescence:

    Article Title: Jumonji domain-containing 6 promotes the expansion of neuroblastoma stem cells by activating the wingless/ integrated pathway
    Article Snippet: The membranes were blocked using 5% non-fat milk and probed with the following primary antibodies: anti-JMJD6 antibody (1:1000, cat. 60602, CST, Danvers, MA, USA), anti-β-Catenin antibody (1:2000, cat. 8480, CST), anti-EF-1α antibody (1:2000, cat. sc-21758, Santa Cruz, Dallas, TX, USA), and anti-GAPDH antibody (1:10000, cat. 2118, CST). .. Then, the membranes were incubated with an horseradish peroxidase-conjugated second antibody (1:10000, cat. 7074, or 1:10000, cat. 7076, CST), and the bands were visualized with electrochemiluminescence luminescence (Bio-Rad, Hercules, CA, USA). ..



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